[RASMB] equilibrium constant

[Neil Errington]

Dear Evan

The conversion is actually (K2 * molar extinction coefficient * path
length)/2

This formula and others for higher order associations can be found in the
book by Beckman:

 Analytical Ultracentrifugation Volume II "Self - Associating Systems in the
Analytical Ultracentrifuge" by  Don McRorie and Paul Voelker. You should be
able to get a copy from your Beckman contacts.
The formulae are doubtless in other publications too but this was the one I
had to hand at the time

All the best

Neil

**************************************************
Dr Neil Errington
Manager, NCMH Business Centre
School of BioSciences
University of Nottingham
Sutton Bonington
Leicestershire.
LE12 5RD. UK

Tel: +44 (0)115 951 6156
Fax: +44 (0)115 951 6157
URL: http://www.nottingham.ac.uk/ncmh/business

*************************************************




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>
>I would appreciate some assistance with the conversion of the equilibrium
>constant (Kd) for monomer <--> dimer from the format generated in winnl106
>(lnK2).
>
>Is the converstion just:
>
>K2 * extinction coefficient of monomer * path length (1.2 cm)
>
>or am I missing something?
>
>Thanks for some assistance.
>
>
>--
>Evan Stein
>Structural Biology Program
>Skirball Institute / NYU School of Medicine
>540 First Avenue Lab 3-4
>New York, NY 10016
>
>phone: (212) 263-8968
>fax: (212) 263-8951
>email: stein at saturn.med.nyu.edu
>URL: http://saturn.med.nyu.edu/~stein
>
>
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